Hawaii INBRE offers Surface Plasmon Resonance (SPR) services and hosts unique Natural Product chemical libraries. Additionally, Kapi’olani Community College in Honolulu specializes in monoclonal antibody production.
Surface Plasmon Resonance (SPR)
Our HI-INBRE partner, Kapi’olani Community College in Honolulu, is equipped with a Nicoya Lifesciences SPR instrument to study biomolecular interactions that are often at the heart of therapeutic discovery. Through quantitative molecule-molecule binding studies, interactive parameters can be derived that form the basis for uncovering new targets and identifying new therapeutic candidates. The digital SPR approach offered by this instrument eliminates the constraints of conventional fluid handling and introduces unprecedented automation capabilities.
Natural Product Collections
As an INBRE based in Hawaii, we seek to uphold our responsibility to share our unique resources, including the enormous potential for the discovery of marine and botanical natural products with pharmacologic value. Hawaii’s relative isolation and climate offer rich potential for natural products researchers and development of applications to disease treatment. At the Hawaii Pacific University, a collection of Marine Natural Products has been established, which have high structural diversity and possess chemical properties that allow them to act as drugs in the human body. At the University of Hawaii at Hilo, a collection has been established of bioactive compounds from Hawaii marine and terrestrial fungi, bacteria, and other microorganisms. Consultation with HI-INBRE partner institutes will be available to determine the potential for making the Natural Product libraries available to RAIN partners. If these projects are deemed feasible with a potential for impactful discovery, a formal collaboration will be established to move these projects forward.
Core Facilities
Data Science Core
RAIN collaborating investigators will be able to access services in research design, data management and bioinformatic data analysis from the Hawaii Data Science Core at the internal rate.
Monoclonal Antibody Core
This service will be available to RAIN state investigators at the internal rate. The monoclonal antibody core facility at Kapiolani Community College specializes in generating and characterizing monoclonal antibodies through hybridoma technology. Through a service contract agreed upon by both parties, this Core will work to provide the requisite biological tools needed by RAIN partners for research, diagnostic, and prognostic uses.
Analytical Chemistry Facilities:
Our two HI-INBRE partners, the University of Hawaii at Hilo Daniel K. Inouye College (DKICOP) of Pharmacy and Hawaii Pacific University (HPU) have sophisticated equipment that is supported by HI-INBRE. These instruments along with the expert faculty directing their use will be available via collaboration:
DKICP
- Bruker Ultra High Shield 400 MHz NMR (Nuclear Magnetic Resonance Spectrometer) with Avance III Console
- Varian MS500 Ion Trap LC-MS (Liquid Chromatography Mass Spectrometer)
- Agilent 6530 Accurate Mass Q-TOF LC-MS (Liquid Chromatography Mass Spectrometer)
- Agilent 7000BTriple Quandrupole GC-MS (Gas Chromatography Mass Spectrometer)
- Agilent 6120 Single Quadrupole LC-MS (Liquid Chromatography Mass Spectrometer)
HPU
- Nuclear Magnetic Resonance Spectrometer: 80 MHz Magritek; probe: 1H and 13C detection with gradients and reaction monitor
- LCMS: Agilent 6530 QTOF mass spectrometer, electrospray and atmospheric pressure chemical ionization, 1200 LC and UV detector
- Evaporative light scattering detector: Shimadzu LT II
- Binary preparative HPLC: Agilent 1100 preparative capable; multi- wavelength UV detector, fraction collector
- FPLC: AKTA Prime
- 2 isocratic HPLC: Shimadzu LC-6A and DStar LC; analytical and semipreparative capable; chart integrator, single wavelength UV detector
- GC-MS: Agilent 7890A/5975C